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Cardiac expression patterns of endothelin-converting enzyme (ECE): Implications for conduction system development
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SYSNO ASEP 0316957 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cardiac expression patterns of endothelin-converting enzyme (ECE): Implications for conduction system development Title Exprese endothelin-konvertujícího enzymu v srdci: implikace pro vývoj převodního systému Author(s) Sedmera, David (UZFG-Y)
Harris, B. S. (US)
Grant, E. (US)
Zhang, N. (US)
Jourdan, J. (US)
Kurková, D. (CZ)
Gourdie, R. G. (US)Source Title Developmental Dynamics. - : Wiley - ISSN 1058-8388
Roč. 237, - (2008), s. 1746-1753Number of pages 8 s. Language eng - English Country US - United States Keywords chick embryo ; heart development ; immunohistochemistry Subject RIV FA - Cardiovascular Diseases incl. Cardiotharic Surgery R&D Projects GA304/08/0615 GA ČR - Czech Science Foundation (CSF) Fellowship J. E. Purkyně GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000256652300022 DOI 10.1002/dvdy.21572 Annotation The spatiotemporal distribution of the endothelin-converting enzyme (ECE) protein in the embryonic chick heart and the association of this polypeptide with the developing cardiac conduction system is described here for the first time. Further, we show how cardiac hemodynamic load directly affects ECE level and distribution. Endothelin (ET) is a cytokine involved in the inductive recruitment of Purkinje fibers. ET is produced by proteolytic cleavage of Big-ET by ECE. We generated an antibody against chick ECE recognizing a single band at approximately 70 kD to correlate the cardiac expression of this protein with that reported previously for its mRNA. ECE protein expression was more widespread compared to its mRNA, being present in endothelial cells, mesenchymal cells, and myocytes, and particularly enriched in the trabeculae and nascent ventricular conduction system. The myocardial expression was significantly modified under experimentally altered hemodynamic loading. In vivo, ET receptor blockade with bosentan delayed activation sequence maturation. These data support a role for ECE in avian cardiac conduction system differentiation and maturation. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2009
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