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Bacterial communities of two contrasting soils reacted differently to lincomycin treatment
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SYSNO ASEP 0314583 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Bacterial communities of two contrasting soils reacted differently to lincomycin treatment Title Bakteriální společenstva dvou různých půd reagovala odlišně na opracovaní linkomycinem Author(s) Čermák, Ladislav (MBU-M)
Kopecký, Jan (MBU-M)
Novotná, Jitka (MBU-M)
Omelka, M. (CZ)
Parkhomenko, Natalia (MBU-M) RID
Plháčková, Kamila (MBU-M) RID
Ságová-Marečková, Markéta (MBU-M)Source Title Applied Soil Ecology. - : Elsevier - ISSN 0929-1393
Roč. 40, č. 2 (2008), s. 348-358Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords bacterial diversity ; forest soils ; lincomycin Subject RIV EE - Microbiology, Virology R&D Projects IAA600200519 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA6020410 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06024 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000259830300016 DOI 10.1016/j.apsoil.2008.06.001 Annotation The antibiotic lincomycin an anthropogenic pollutant –may change the bacterial diversity and resistance of soil communities by increasing selection pressure in favour of resistant phenotypes. In a laboratory experiment, two forest soils of contrasting characteristics in both pH and clay content were examined for changes in their bacterial communities after lincomycin treatment. The two soils were incubated in beakers and treated with three dosages of lincomycin. Changes in bacterial community were assessed by (i) cultivation of bacteria on two different media, and percentage of resistant colonies at two lincomycin concentrations;(ii) analysis of putative actinomycete isolates for antibiotic activities and resistance; (iii) TRFLP diversity profiles of the 16S rDNA and homologues of the resistance gene lmrB; and (iv) cloning-sequencing of PCR amplicons of both 16S rDNA and lmrB homologues Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2009
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