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Protein signaling pathways in differentiation of neural stem cells
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SYSNO ASEP 0313817 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Protein signaling pathways in differentiation of neural stem cells Title Proteinové signální dráhy řídící diferenciaci nervových kmenových buněk Author(s) Skalníková, Helena (UZFG-Y) RID, ORCID
Vodička, Petr (UZFG-Y) ORCID
Pelech, S. (CA)
Motlík, Jan (UZFG-Y) RID, ORCID
Gadher, S. J. (CH)
Kovářová, Hana (UZFG-Y) RID, ORCIDSource Title Proteomics. - : Wiley - ISSN 1615-9853
Roč. 8, - (2008), s. 4547-4559Number of pages 13 s. Language eng - English Country DE - Germany Keywords antibody microarray ; differentiation ; neural stem cells Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0538 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000261003800015 DOI https://doi.org/10.1002/pmic.200800096 Annotation Neural stem cells (NSC) are promising source of cells for the treatment of central nervous system diseases. Access to regulatory signaling proteins present in low copies has been very restrictive until now due to scarcity of stem cell populations. Hence, we utilized the KinexTM Antibody Microarray analysis where profiles of the proliferating porcine NSC and differentiated counterparts were compared. Selected changes were verified by immunoblotting. Differentiated neural cells exhibited an increased level of RafB kinase, MAPKK 3, heme oxygenase 2 (HO2) and protein phosphatase 4C. In contrast, for the NSC were typical high level of G protein-coupled receptor-serine kinase 2 and enhanced phosphorylations of B-crystallin (S45), PKC (T655), PKC (S738/S742) together with eIF2 (S51). Immunocytochemistry confirmed expression profiles and intense cytoplasmic localization of HO2 in neurons but a weaker signal in glia. Phospho-S45 B-crystallin was localized in nuclei of differentiated cells. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2009
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