Two-dimensional electrophoretic comparison of metastatic and non-metastatic human breast tumors using in vitro cultured epithelial cells derived from the cancer tissues

Vydra, J.; Selicharová, Irena; Smutná, Kateřina; Šanda, Miloslav; Matoušková, Eva; Buršíková, Eva; Prchalová, Markéta; Velenská, Z.; Coufal, David; Jiráček, Jiří

doi:https://dx.doi.org/10.1186/1471-2407-8-107

Number of the records: 1

Two-dimensional electrophoretic comparison of metastatic and non-metastatic human breast tumors using in vitro cultured epithelial cells derived from the cancer tissues

1.

SYSNO ASEP	0311358
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Two-dimensional electrophoretic comparison of metastatic and non-metastatic human breast tumors using in vitro cultured epithelial cells derived from the cancer tissues
Title	Porovnání buněčných kultur epitelových buněk získaných z metastázujících a nemetastázujících nádorů prsu pomocí dvojrozměrná elektroforetické analýzy
Author(s)	Vydra, J. (CZ) Selicharová, Irena (UOCHB-X)_{RID, ORCID} Smutná, Kateřina (UOCHB-X) Šanda, Miloslav (UOCHB-X) Matoušková, Eva (UMG-J) Buršíková, Eva (UMG-J) Prchalová, Markéta (UMG-J) Velenská, Z. (CZ) Coufal, David (UIVT-O)_{RID, SAI, ORCID} Jiráček, Jiří (UOCHB-X)_{RID, ORCID}
Source Title	BMC Cancer. - : BioMed Central - ISSN 1471-2407 Roč. 8, č. 107 (2008), s. 1-13
Number of pages	13 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	breast cancer ; 2-DE ; metastases
Subject RIV	CE - Biochemistry
R&D Projects	NR8323 GA MZd - Ministry of Health (MZ)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
	AV0Z10300504 - UIVT-O (2005-2011)
UT WOS	000255938700001
DOI	https://doi.org/10.1186/1471-2407-8-107
Annotation	We prepared primary cultures of epithelial cells from 23 breast cancer tissue samples and performed comparative proteomic analysis using 2-DE. Seven patients developed distant metastases and formed a metastase-positive group, the others formed a metastase-negative group. Spot densities in 2-DE protein maps were subjected to statistical analyses (R/maanova package) and data-mining analysis (GUHA). Proteins were identified by LC-MS/MS. Three protein spots were significantly altered between the metastatic and non-metastatic groups.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
Year of Publishing	2009

Number of the records: 1