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Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate
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SYSNO ASEP 0310855 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate Title Analýza enantiomerů aminokyselin v peptidu Carbetocinu plynovou chromatografií po derivatizaci pentafluorpropylchlormravenčanem Author(s) Zahradníčková, Helena (BC-A) RID, ORCID
Hartvich, Petr (BC-A)
Šimek, Petr (BC-A) RID, ORCID
Hušek, Petr (BC-A)Number of authors 4 Source Title Amino Acids. - : Springer - ISSN 0939-4451
Roč. 35, č. 2 (2008), s. 445-450Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords D,L-amino acids ; peptides ; chloroformates Subject RIV CC - Organic Chemistry R&D Projects GA203/04/0192 GA ČR - Czech Science Foundation (CSF) GA303/06/1674 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000258523700027 DOI https://doi.org/10.1007/s00726-007-0577-1 Annotation A novel sample preparation protocol for gas chromatographic (GC) analysis of amino acid enantiomers in peptides was developed. It comprises traditional acid hydrolysis, a novel treatment of the analytes with a fluoroalkyl chloroformate and GC/FID separation of enantiomers on a chiral capillary column. The major improvements consist in that the derivatization step proceeds in organic- aqueous media within seconds and the amino acid derivatives are volatile enough to suit the temperature range of the chiral Chirasil-Val capillary column. The approach was found beneficial for chiral analysis of pharmaceutically important Carbetocin peptide. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2009
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