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Kinetics and Specificity of Lipozyme-Catalysed Oil Hydrolysis in Supercritical CO2
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SYSNO ASEP 0310266 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Kinetics and Specificity of Lipozyme-Catalysed Oil Hydrolysis in Supercritical CO2 Title Kinetika a specificita hydrolýzy oleje v superkritickém CO2 katalyzované Lipozymem Author(s) Sovová, Helena (UCHP-M) RID, ORCID, SAI
Zarevúcka, Marie (UOCHB-X) RID
Bernášek, Prokop (UCHP-M)
Stamenič, M. (CZ)Source Title Chemical Engineering Research and Design. - : Elsevier - ISSN 0263-8762
Roč. 86, č. 7 (2008), s. 673-681Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords blackcurrant seed oil ; enzymatic hydrolysis ; supercritical CO2 Subject RIV CI - Industrial Chemistry, Chemical Engineering R&D Projects GA104/06/1174 GA ČR - Czech Science Foundation (CSF) OC D30.001 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40720504 - UCHP-M (2005-2011) AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000258545800003 DOI https://doi.org/10.1016/j.cherd.2008.03.006 Annotation Selective enzyme-catalysed hydrolysis of blackcurrant seed oil was studied with aim to obtain different levels of alpha- and/or gamma-linolenic acid in lipid classes in the hydrolyzate, making them suitable for special dietary needs. Supercritical CO2 with dissolved oil and water flowed through a packed bed reactor (temperature 40 °C, pressure 15-28 MPa, superficial velocity 0.1-0.7 mm/s) where Lipozyme, a 1,3-specific lipase from Mucor miehei was immobilised on a macroporous ion-exchange resin. The enzyme activity was stable as long as water precipitation in the reactor was prevented. The reaction was controlled by both Michaelis-Menten kinetics and mass transfer. The maximum rate of fatty acids liberation per unit amount of enzyme, 2.6 mmol/s kg, was achieved at the maximum pressure and flow rate. The liberated fatty acids were enriched in alpha-linolenic, palmitic and stearic acids, while di- and monoacylglycerols contained increased levels of gamma-linolenic and stearidonic acids. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2009
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