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Gas chromatographic-mass spectrometric analysis of biomarkers related to folate and cobalamin status in human serum after dimercaptopropanesulfonate reduction and heptafluorobutyl chloroformate derivatization
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SYSNO ASEP 0310218 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Gas chromatographic-mass spectrometric analysis of biomarkers related to folate and cobalamin status in human serum after dimercaptopropanesulfonate reduction and heptafluorobutyl chloroformate derivatization Title Analýza biomarkrů folátového a kobalaminového stavu v lidském séru po redukci dimerkaptopropansulfonátem a derivatizaci heptafluorobutylchlormravenčanem plynovou chromatografií s hmotnostní detekcí Author(s) Šimek, Petr (BC-A) RID, ORCID
Hušek, Petr (BC-A)
Zahradníčková, Helena (BC-A) RID, ORCIDNumber of authors 3 Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 80, č. 15 (2008), s. 5776-5782Number of pages 7 s. Language eng - English Country US - United States Keywords biomarkers ; gas chromatographic ; chloroformate derivatization Subject RIV CB - Analytical Chemistry, Separation R&D Projects GA303/06/1674 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000258096700020 DOI https://doi.org/10.1021/ac8003506 Annotation Methylmalonic acid and total homocysteine belong to useful clinical indicators of cobalamin and folate status and are commonly measured separately. A sensitive and rapid method has been developed for simultaneous determination of both biomarkers and related metabolites in serum or plasma by isotope dilution gas chromatography–mass spectrometry. Thiols bound in disulfide bonds were released with 2, 3-dimercapto-1-propanesulfonic acid (DMPS), and after deproteinization, they were phase-transfer derivatized with heptafluorobutyl chloroformate in a single step. The reducing capability of the DMPS agent was comparable to that of the dithiothreitol, but exceeded the latter in much cleaner extracts obtained. The new method enabled GC-MS screening of aminoacidic metabolites as their N(S,O)-heptafluorobutoxycarbonyl heptafluorobutyl ester derivatives in serum or plasma. Accurate quantitation of seven biomarkers was accomplished by using deuterated internal standards. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2009
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