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VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation
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SYSNO ASEP 0308384 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation Title VDAC2 a aldoláza A identifikovány jako membránové proteiny buněk K562 se zvýšenou expresí za podmínek deprivace železa Author(s) Vališ, Karel (BTO-N)
Neubauerová, J. (CZ)
Man, Petr (MBU-M) RID, ORCID
Pompach, Petr (MBU-M) RID, ORCID
Vohradský, Jiří (MBU-M) RID, ORCID
Kovář, J. (CZ)Source Title Molecular and Cellular Biochemistry. - : Springer - ISSN 0300-8177
Roč. 311, 1-2 (2008), 225-231Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords Iron deprivation ; aldolase A ; VDAC2 Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50520701 - BTO-N (2007-2013) AV0Z50200510 - MBU-M (2005-2011) Annotation We have shown previously that iron deprivation significantly stimulates the uptake of non-transferrin ferric iron from ferric citrate by erythroleukemia K562cells and that this stimulation depends on protein synthesis. However, we have not detected increased expression of any known iron transport protein. Therefore, in order to identify membrane proteins of K562 cells with increased expression under iron deprivation, we employed the isolation of membrane proteins by two-phase partitioning system, protein separation by two-dimensional electrophoresis, computer differential analysis, and tandem mass spectrometry. Thus, we identified two proteins with statistically significant upregulation, i.e., aldolase A and voltage-dependent anion channel 2 (VDAC2). The upregulation of aldolase A and VDAC2 in K562 cells under iron deprivation was also confirmed by western blot analysis. This is the first time when the control of aldolase A and VDAC2 levels by iron status of the cell is demonstrated Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2008
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