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Aminophenyl- and nitrophenyl-labeled nucleoside triphosphates. Synthesis, enzymatic incorporation and electrochemical detection
- 1.0306011 - ÚOCHB 2008 RIV DE eng J - Journal Article
Cahová, Hana - Havran, Luděk - Horáková Brázdilová, Petra - Pivoňková, Hana - Pohl, Radek - Fojta, Miroslav - Hocek, Michal
Aminophenyl- and nitrophenyl-labeled nucleoside triphosphates. Synthesis, enzymatic incorporation and electrochemical detection.
[Aminofenyl- and nitrofenyl-značené nukleosid trifosfáty. Syntéza, enzymatické inkorporace a electrochemická detekce.]
Angewandte Chemie - International Edition. Roč. 47, č. 11 (2008), s. 2059-2062. ISSN 1433-7851. E-ISSN 1521-3773
R&D Projects: GA ČR GA203/05/0043; GA ČR GA203/07/1195; GA MŠMT LC512; GA MŠMT(CZ) LC06035
Institutional research plan: CEZ:AV0Z40550506; CEZ:AV0Z50040507
Keywords : nucleotides * cross-coupling reactions * DNA * polymerases
Subject RIV: CC - Organic Chemistry
Impact factor: 10.879, year: 2008
Aminophenyl- and nitrophenyl-labeled nucleoside triphosphates were prepared by aqueous cross-coupling reactions and were incorporated to DNA by polymerase. The resulting labeled DNA was used for electrochemical detection and bioanalytical applications (e.g. minisequencing).
Aminofenyl- and nitrofenyl-značené nukleosid trifosfáty byly připraveny vodnými cross-coupling reakcemi a byly inkorporovány do DNA polymerasou. Výsledné značené DNA byly využity pro elektrochemickou detekci a bioanalytické aplikace (např. minisekvenování).
Permanent Link: http://hdl.handle.net/11104/0159122
Number of the records: 1