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Detection of apoptosis in paraffin embedded tissues: the influence of tissue type and fixation
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SYSNO ASEP 0213494 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Detection of apoptosis in paraffin embedded tissues: the influence of tissue type and fixation Author(s) Matalová, Eva (UZFG-Y) RID
Dubská, L. (CZ)
Míšek, Ivan (UZFG-Y)Source Title Acta veterinaria Brno. - : Veterinární a farmaceutická univerzita Brno - ISSN 0001-7213
Roč. 71, č. 4 (2002), s. 529-533Number of pages 5 s. Language eng - English Country CZ - Czech Republic Keywords apoptosis ; TUNEL test ; paraffin embedded tissues Subject RIV EB - Genetics ; Molecular Biology R&D Projects GP204/02/P112 GA ČR - Czech Science Foundation (CSF) KSK6005114 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Annotation The influence of different ways of fixation on TUNEL POD results was investigated. Three commonly employed fixative procedures using formol, methacarn and Bouin liquid were applied. Two bovine tissue types with different cell density (thymus and ovary) were used to test this effect. Different pretreatments were tested in case of false negative findings. Results were evaluated under light microscopy and photodocumented. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2003
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