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Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes
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SYSNO ASEP 0213221 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes Author(s) Motlík, Jan (UZFG-Y) RID, ORCID
Pavlok, Antonín (UZFG-Y) RID
Lapathitis, Georgios (UZFG-Y)
Kubelka, Michal (UZFG-Y) RID, ORCIDSource Title Reproduction in Domestical Animals. - : Wiley - ISSN 0936-6768
Roč. 35, - (2000), s. 267-271Number of pages 5 s. Language eng - English Country US - United States Subject RIV EB - Genetics ; Molecular Biology R&D Projects GV524/96/K162 GA ČR - Czech Science Foundation (CSF) KSK2038602 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Annotation Proper conditions for the successful induction of a functional two step in vitro culture system for agricultural or clinical applications are hereby described in form of a two-step protocol. The first inhibitory culture of oocytes in media supplemented with specific cdk inhibitors (roscovitine, BL I) appears essential to promote appropriate oocyte-cumulus cell interactions and ensure production of ova competent to undergo normal embryogenesis. This inhibitory period should last for several days, depending on the origin of the follicles, and the degree of accumulation and storage of mRNAs and proteins essential for the acquisition of developmental competence in the oocytes. The full reversibility of the inhibitory step secures that during the second maturation step, oocytes reach metaphase IT with correctly arranged microtubuli in the meiotic spindle and well-expanded cumuli. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2001
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