P-selectin glycoprotein ligand-1 and E-selectin ligand-1 are differentially modified by fucosyltransferases Fuc-TIV and Fuc-TVII in mouse neutrophils

0191184 - UMG-J 20010254 RIV US eng J - Journal Article
Huang, M. Ch. - Zollner, O. - Moll, T. - Malý, Petr - Thall, A. D. - Lowe, J. B. - Vestweber, D.
P-selectin glycoprotein ligand-1 and E-selectin ligand-1 are differentially modified by fucosyltransferases Fuc-TIV and Fuc-TVII in mouse neutrophils.
Journal of Biological Chemistry. Roč. 275, č. 40 (2000), s. 31353-31360. ISSN 0021-9258. E-ISSN 1083-351X
Institutional research plan: CEZ:AV0Z5052915
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 7.368, year: 2000

We used mice deficient in Fuc-TIV and/or Fuc-TVII to examinehow these enzymes generate selectin-binding glycoforms of PSGL-1 and ESL-1 in mouse neutrophils using recombinant antibody-like forms of the respective endothelial selectins. Our data imply that in mouse neutrophils and their precursors, Fuc-TVII exclusively directs expression of PSGL-1 glycoforms bound with high affinity by P-selectin, while Fuc-TIV preferentially directs expression of ESL-1 glycoforms that exhibit high affinity for E-selectin.
Permanent Link: http://hdl.handle.net/11104/0086941