0181611 - UFCH-W 20030040 RIV GB eng J - Journal Article
Smith, D. - Wang, T. - Sule-Suso, J. - Španěl, Patrik - El Haj, A.
Quantification of Acetaldehyde Released by Lung Cancer Cells in vintro Using Selected Ion Flow Tube Mass Spectrometry.
Rapid Communications in Mass Spectrometry. Roč. 17, - (2003), s. 845-850. ISSN 0951-4198. E-ISSN 1097-0231
R&D Projects: GA ČR GA202/03/0827; GA ČR GA203/02/0737
Institutional research plan: CEZ:AV0Z4040901
Keywords : stage ranal-failure * trace gas-analysis * quantitative-analysis
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 2.789, year: 2003

The production of volatile compounds from cancer cell lines in vitro has been investigated using selected ion flow tube mass spectrometry (SIFT-MS). This technique enables on-line quantitative analyses of the headspace above cell/medium cultures. This paper reports the discovery that acetaldehyde is released by the lung cancer cell lines SK-MES and CALU-1. The concentration of acetaldehyde in the headspace of the medium/cell culture was measured after 16h incubation at 37degreesC and found to be proportional to the number of cancer cells in the medium (typically 10(8)). From these data, the acetaldehyde production rates of the SK-MES cells and the CALU-1 cells in vitro are determined to be 1 x 10(6) and 1.5-3 x 10(6) molecules/cell/min, respectively. The potential value of this new technique in cell biology and in industrial cell biotechnology is discussed.
Permanent Link: http://hdl.handle.net/11104/0001917