0181593 - UFCH-W 20030020 RIV CH eng J - Journal Article
Hromadová, Magdaléna - Salmain, M. - Sokolová, Romana - Pospíšil, Lubomír - Jaouen, G.
Novel Redox Label for Proteins. Electron Transfer Properties of (.eta.5-cyclopentadienyl) Tricarbonyl Manganese Bound to Bovine Serum Albumin.
Journal of Organometallic Chemistry. Roč. 668, 1/2 (2003), s. 17-24. ISSN 0022-328X. E-ISSN 1872-8561
R&D Projects: GA ČR GP203/02/P082; GA MŠMT OC D15.10
Institutional research plan: CEZ:AV0Z4040901
Keywords : cymantrene * redox label * AC voltammetry
Subject RIV: CG - Electrochemistry
Impact factor: 2.042, year: 2003

(.eta.5cyclopentadienyl) tricarbonyl manganese (cymantrenyl) derivatives of the protein bovine serum albumin (BSA) with coupling ratios ranging from 7 to 20 were prepared by reaction of cymantrenyl methyl imidate with some of the lysine residues of BSA. Electrochemical measurements based on the AC voltammetric detection of the reduction of cymantrene bound to BSA protein were successfully set up in aqueous solutions and the detection limit of 2 107 M BSA was achieved. This limit is almost two orders of magnitude lower than that measured by IR spectroscopy. Electrochemical detection of cymantrene label will be used for immunoassay analysis of waterůsoluble analytes in our future work.
Permanent Link: http://hdl.handle.net/11104/0078136