0181508 - UFCH-W 20020246 RIV CZ eng J - Journal Article
Häfner, A. - Mérola, F. - Duportail, G. - Schneider, F. W. - Hof, Martin - Beneš, Martin
Time-resolved Tryptophan Fluorescence of Fragment 1-86 of Factor X and the Influence of Membrane Binding.
Collection of Czechoslovak Chemical Communications. Roč. 67, - (2002), s. 1872-1882. ISSN 0010-0765
R&D Projects: GA MŠMT LN00A032
Institutional research plan: CEZ:AV0Z4040901
Keywords : protein fluorescence * factor Xa * Gla domain
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 0.848, year: 2002

Membrane binding of the N-terminus of the prothrombinase reaction enzyme, fragment 1-86 of factor X, to phospholipid vesicles was studied using time-resolved fluorescence spectroscopy of the tryptophan residue Trp41 which is located in the membrane binding part of this protein (Gla domain). Fluorescence lifetimes were determined by the time-correlated single photon couting technique using synchrotron radiation as the excitation source.
Permanent Link: http://hdl.handle.net/11104/0078067