0173629 - UEM-P 20023084 RIV US eng J - Journal Article
Malínský, Jan - Koberna, Karel - Bednár, J. - Štulík, J. - Raška, Ivan
Searching for active ribosomal genes in situ:light microscopy in light of the electron beam.
Journal of Structural Biology. Roč. 140, - (2002), s. 227-231. ISSN 1047-8477. E-ISSN 1095-8657
R&D Projects: GA ČR GA304/00/1481; GA ČR GA304/01/0729; GA ČR GA304/00/D035; GA AV ČR IAA5039103
Institutional research plan: CEZ:AV0Z5039906; CEZ:MSM 111100003
Keywords : fluorescence * image restoration * ribosomal genes
Subject RIV: EA - Cell Biology
Impact factor: 4.194, year: 2002

To improve the LM resolution, computational methods based on removal of the image blur are frequently implemented in cell biology studies. One of the standard deblurring approaches is image restoration throught deconvolution algorithms. We demonstrate that, in the specific case of the fluorescence mapping of active ribosomal genes in HeLa cell nucleoli, restoration generates a biased result. On restoration of model images, we demostrate the difficulties of one of the most effective deconvolution algorithms during the restoration of ring-shaped fluorescent objects of a diameter comparable to the microscope resolution limit.
Permanent Link: http://hdl.handle.net/11104/0070658