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Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of ("Christmas trees)" in situ
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SYSNO ASEP 0173594 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of ("Christmas trees)" in situ Author(s) Koberna, Karel (UEM-P)
Malínský, Jan (UEM-P) RID, ORCID
Pliss, Artem (UEM-P)
Mašata, Martin (UEM-P)
Večeřová, Jaromíra (UEM-P)
Fialová, Markéta (UEM-P)
Bednár, J. (FR)
Raška, Ivan (UEM-P)Source Title Journal of Cell Biology. - : Rockefeller University Press - ISSN 0021-9525
Roč. 157, č. 5 (2002), s. 743-748Number of pages 6 s. Language eng - English Country US - United States Keywords cell nucleolus ; rRNA genes ; ribosomal RNA Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA304/00/1481 GA ČR - Czech Science Foundation (CSF) GA304/01/0729 GA ČR - Czech Science Foundation (CSF) GA304/00/D035 GA ČR - Czech Science Foundation (CSF) IAA5039103 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z5039906 - UEM-P MSM 111100003 Annotation The organization of transcriptionally active ribosomal genes in animal cell nucleoli is investigated in this study. We demonstrate that each nucleolar focus observed by LM corresponds, on the EM level, to an individual fibrillar center (FC) and surrounding dense fibrillar components (DFCs). The EM data identify the DFC as the nucleolar subcompartment in which rRNA synthesis takes place. Intense and unambiguous clustered immunogold signal of nascent transcripts is strongly indicative of nascent 'Christmas trees' of rRNA associated with individual rDNA genes. Workplace Institute of Experimental Medicine Contact Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2003
Number of the records: 1