0173594 - UEM-P 20023042 RIV US eng J - Journal Article
Koberna, Karel - Malínský, Jan - Pliss, Artem - Mašata, Martin - Večeřová, Jaromíra - Fialová, Markéta - Bednár, J. - Raška, Ivan
Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of ("Christmas trees)" in situ.
Journal of Cell Biology. Roč. 157, č. 5 (2002), s. 743-748. ISSN 0021-9525. E-ISSN 1540-8140
R&D Projects: GA ČR GA304/00/1481; GA ČR GA304/01/0729; GA ČR GA304/00/D035; GA AV ČR IAA5039103
Institutional research plan: CEZ:AV0Z5039906; CEZ:MSM 111100003
Keywords : cell nucleolus * rRNA genes * ribosomal RNA
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 12.523, year: 2002

The organization of transcriptionally active ribosomal genes in animal cell nucleoli is investigated in this study. We demonstrate that each nucleolar focus observed by LM corresponds, on the EM level, to an individual fibrillar center (FC) and surrounding dense fibrillar components (DFCs). The EM data identify the DFC as the nucleolar subcompartment in which rRNA synthesis takes place. Intense and unambiguous clustered immunogold signal of nascent transcripts is strongly indicative of nascent 'Christmas trees' of rRNA associated with individual rDNA genes.
Permanent Link: http://hdl.handle.net/11104/0070624