0173415 - UEM-P 20010011 RIV US eng J - Journal Article
Melčák, Ivo - Melčáková, Štěpánka - Kopský, Vojtěch - Večeřová, Jaromíra - Raška, Ivan
Prespliceosomal Assembly on Micoinjected Precursor mRNA Takes Place in Nuclear Speckles.
Molecular Biology of the Cell. Roč. 12, - (2001), s. 393-406. ISSN 1059-1524. E-ISSN 1939-4586
R&D Projects: GA ČR GA302/99/0587; GA ČR GA304/00/1481; GA MŠMT VS96129
Institutional research plan: CEZ:AV0Z5039906
Keywords : splicing nuclear speckles
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 7.700, year: 2001

We show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA (pre-mRNA) is associated with the speckles. We conclude that the (pre)spliceosomal complexs on microinjected pre-mRNA are formed inside the speckles. Their targeting into and accumulation in the speckles is a result of the cumulative loading of splicing factors to the pre-mRNA and the complexes formed give rise to the speckled pattern observed.
Permanent Link: http://hdl.handle.net/11104/0070448