0173378 - UEM-P 20003102 RIV CZ eng C - Conference Paper (international conference)
Staněk, David - Koberna, Karel - Pliss, Artem - Čtrnáctá, Vlasta - Malínský, Jan - Mašata, Martin - Večeřová, Jaromíra - Raška, Ivan
Transport of ribosomal RNA within the nucleolus.
EUREM 12. Brno: organizator symposia, 2000, s. B 293.
[EUREM /12./. Brno (CZ), 09.07.2000-14.07.2000]
R&D Projects: GA MŠMT VS96129; GA ČR GA304/00/P025; GA ČR GA304/00/1481
Grant - others:Wellcome Trust grant(XX) 049949/Z/97/Z/JMW/JPS/CG
Institutional research plan: CEZ:AV0Z5039906
Subject RIV: EB - Genetics ; Molecular Biology

In the present study, we have explored the fact that incorporation of BrU into prerRNA did not interfere with pre-rRNA processing (11) and we have investigated the transport of non - isotopically labeled RNA within the nucleolus by means of transmissiion electron microscopy and confocal laser scanning microscopy.Within 20 minutes, BrU - labeled nucleolar RNA moved from the transcription sites in nucleolar DFC to GC. Double localization of bromouridine labeled RNA and fibrillarin revealed that only a ppart of the fibrillarin rich domains were transcriptionally active.
Permanent Link: http://hdl.handle.net/11104/0070411