The yeast Ura2 protein that catalyses the first two steps of pyrimidines biosynthesis accumulates not in the nucleus but in the cytoplasm, as shown by immunocytochemistry and Ura2 - green fluorescent protein mapping

Name: The yeast Ura2 protein that catalyses the first two steps of pyrimidines biosynthesis accumulates not in the nucleus but in the cytoplasm, as shown by immunocytochemistry and Ura2 - green fluorescent protein mapping
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Benoist, P.; Feau, P.; Pliss, Artem; Voříšek, Josef; Antonelli, R.; Raška, Ivan; Duphil, M. D.

Number of the records: 1

The yeast Ura2 protein that catalyses the first two steps of pyrimidines biosynthesis accumulates not in the nucleus but in the cytoplasm, as shown by immunocytochemistry and Ura2 - green fluorescent protein mapping

1.

SYSNO ASEP	0173346
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	The yeast Ura2 protein that catalyses the first two steps of pyrimidines biosynthesis accumulates not in the nucleus but in the cytoplasm, as shown by immunocytochemistry and Ura2 - green fluorescent protein mapping
Author(s)	Benoist, P. (ES) Feau, P. (FR) Pliss, Artem (UEM-P) Voříšek, Josef (MBU-M) Antonelli, R. (FR) Raška, Ivan (UEM-P) Duphil, M. D. (FR)
Source Title	Yeast - ISSN 0749-503X Roč. 16, - (2000), s. 1299-1312
Number of pages	14 s.
Language	eng - English
Country	NL - Netherlands
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA302/99/0587 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z5039906 - UEM-P
Annotation	The Ura2 protein was believed to be nucleoplasmic by means of three alternative approaches based on the detection of the protein itself: (a) indirect immunofluorescence of yeast protoplasts: (b) immunogold labelling of ultrathin sections of embedded yeast cells and (c) direct fluorescence of cells ewpressing an Ura2 - green fluorescent protein hybrid localize the bulk of Ura2 to the cytoplasm.
Workplace	Institute of Experimental Medicine
Contact	Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Year of Publishing	2001

Number of the records: 1