Bioassays in Plant Cells for Improvement of Ecosystem and Human Health. - Katowice, 2003
Pages
s. 123-130
Number of pages
8 s.
Action
Bioassays in Plant Cells
Event date
15.05.2003
VEvent location
Katowice
Country
PL - Poland
Event type
WRD
Language
eng - English
Country
PL - Poland
Keywords
DNA damage ; genotoxicity
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
GA521/02/0400 GA ČR - Czech Science Foundation (CSF)
LN00B030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ
AV0Z5038910 - UEB-Q
Annotation
Plants are exposed to various types of environmental xenobiotics, either deliberately as in the case of agricultural pesticides and plant growth regulators, or accidentally as compounds present in polluted air, soil or water. Assays to detect the genotoxicity of these xenobiotics are at present not available for most plant species. This limitation hampers or prevents the detection of the genotoxicity of environmental xenobiotics in plants growing, for example, on polluted soil. To overcome this limitation, a plant-based molecular assay - the COMET or Single Cell Gel Electrophoresis (SCGE) assay, can be applied to detect induced DNA damage (see reviews TICE et at, 2000; COLLINS, 2002). Although this technique has been primarily applied to animal cells, the incorporation of the Comet assay with plant tissues (KOPPEN, VERSCHAEVE, 1996; GICHNER, PLEWA, 1998) significantly extends the utility of plants in basic and applied studies in environmental mutagenesis. In theory, the Comet assay can be used to every plant species.
Workplace
Institute of Experimental Botany
Contact
David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing
2004
Number of the records: 1
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