0172418 - UEB-Q 20033145 RIV SIGLE FR eng C - Conference Paper (international conference)
Vaňková, Radomíra - Kuncová, Gabriela - Podrazký, Ondřej - Gaudinová, Alena - Vaněk, Tomáš
Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy.
Proceedings. France: Bioencapsulation Research Group, 2003, s. 1-4.
[International Workshop on Bioencapsulation /11./. Illkirch (FR), 25.03.2003-27.03.2003]
R&D Projects: GA MŠMT OC 840.20
Institutional research plan: CEZ:AV0Z4072921; CEZ:AV0Z5038910
Keywords : fluorescence spectroscopy * immobilization * dimethyl sulfoxide
Subject RIV: CE - Biochemistry

Using two-dimensional fluorescence spectroscopy, the growth of tobacco cells immobilized in alginate and pectate was monitored during the cultivation of the beads via the fluorescence of proteins (especially of tryptophan). Cell viability was followed by determination of their esterase activity using fluorescein diacetate as a substrate. This technique enables to measure the fluorescence of two fluorophores simultaneously. The effect of several stresses, (namely high and low temperature and the presence of organic solvent dimethylsulfoxide) was followed. Suitability of this technique to monitor the metabolic activities of cells within the beads was demonstrated.
Permanent Link: http://hdl.handle.net/11104/0069457