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Comparison of cytokinin-binding proteins from wheat and oat grains
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SYSNO ASEP 0172370 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Comparison of cytokinin-binding proteins from wheat and oat grains Author(s) Kamínek, Miroslav (UEB-Q) RID, ORCID
Trčková, M. (CZ)
Fox, J. E. (US)
Gaudinová, Alena (UEB-Q) ORCIDSource Title Physiologia Plantarum. - : Wiley - ISSN 0031-9317
Roč. 117, č. 4 (2003), s. 453-458Number of pages 6 s. Language eng - English Country DK - Denmark Keywords Cytokinin-binding proteins ; Avena sativa ; SDS-polyacrylamide gel Subject RIV EB - Genetics ; Molecular Biology R&D Projects GV206/96/K188 GA ČR - Czech Science Foundation (CSF) GA522/02/0530 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z5038910 - UEB-Q Annotation Cytokinin-binding proteins (CBPs) isolated from mature grains of oat (Avena sativa L.) and wheat (Triticum aestivum L.) by acid precipitation, ion-exchange and affinity chromatography had similar characteristics, although they differed somewhat in apparent molecular weight of the native protein as determined by gel filtration (109 and 133 kDa, respectively) and subunit size as estimated by SDS-polyacrylamide gel electrophoresis (47 and 55 kDa, respectively). Highly purified oat CBP showed very weak but distinct immunochemical cross-reactivity with anti-wheat CBP IgG, indicating different immunogenic properties of the two CBPs. Nevertheless, both CBPs exhibited very similar binding of different cytokinins and were characterized by high affinity for N6-benzyladenine (BA)-type and by low affinity for zeatin-type cytokinins to both wheat and oat CBPs and by somewhat higher binding activities of oat CBP compared to wheat CBP (Kds for BA: 4.6 107 M and 6.8 107 M, respectively). The potential role of CBPs in regulating free BA-type cytokinin levels during cereal grain development and germination is discussed. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2004
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