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Antiapoptotic activity of synthetic and natural peptides

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    SYSNO ASEP0172359
    Document TypeC - Proceedings Paper (int. conf.)
    R&D Document TypeConference Paper
    TitleAntiapoptotic activity of synthetic and natural peptides
    Author(s) Franěk, František (UEB-Q)
    Source Title11th Euroconference on Apoptosis "Cell death under the three towers". - : European Cell Death Organisation, 2003 / Vandenabeele P. ; Declercq W.
    Pagess. 33
    Number of pages1 s.
    ActionEuroconference on Apoptosis /11./
    Event date25.10.2003-28.10.2003
    VEvent locationGhent
    CountryBE - Belgium
    Event typeWRD
    Languageeng - English
    CountryBE - Belgium
    KeywordsApoptosis ; hybridoma ; size-exclusion chromatography
    Subject RIVCE - Biochemistry
    R&D ProjectsGA203/01/0083 GA ČR - Czech Science Foundation (CSF)
    CEZAV0Z5038910 - UEB-Q
    AnnotationOur previous studies have shown that peptide mixtures separated from protein digests, as well as individual synthetic peptides are capable of significantly delaying the onset of apoptosis that occurs spontaneously at the decline phase of cell cultures (Franek et al., Biotechnol. Prog.18:155 (2002), Biotechnol. Prog.19:169 (2003)). The characteristic feature of the action of small peptides (two to six amino acid residues) on hybridoma, cultures was the millimolar concentration range needed to achieve significanFchanges of culture parameters. The observed favourable effects of peptides could not be explained by improvement of cell nutrition, because mixtures of free amino acids, constituting the peptides, did not exert comparable effects. The variety of peptides tested for their anti-apoptotic activity in mouse hybridoma cultures was now extended to peptides containing non-coded amino acids and D-amino acids. The apoptotic index Al (i.e. apoptotic cells / total cells) in control cultures at day 6 was (46+-4). The anti-apoptotic effect of D-trialanine was found to be comparable to the effect of L-trialanine, i.e. Al 20 and Al 22, respectively. In the culture supplemented with tri-beta-alanine the value of Al was 29.
    WorkplaceInstitute of Experimental Botany
    ContactDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Year of Publishing2004

Number of the records: 1  

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