24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
1.
SYSNO ASEP
0172341
Document Type
G - Proceedings (int. conf.)
R&D Document Type
Není vybrán druh dokumentu
Title
24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
Author(s)
Franěk, František (UEB-Q) Kohout, Ladislav (UOCHB-X) Eckschlager, T. (CZ)
Issue data
Asociace českých chemických společností, 2003
Number of pages
1 s.
Action
Conference of Isoprenoids /20./
Event date
12.10.2003-18.10.2003
VEvent location
Liberec
Country
CZ - Czech Republic
Event type
WRD
Language
eng - English
Country
CZ - Czech Republic
Keywords
Brassinolides ; cell division
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
IBS4055304 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
GA203/01/0083 GA ČR - Czech Science Foundation (CSF)
OC 844.10 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ
AV0Z4055905 - UOCHB-X
AV0Z5038910 - UEB-Q
Annotation
Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured mammalian cells. A mouse hybridoma, i.e. a hybrid ymphocyte cell line, was cultured either in standard serum-free medium, or in diluted medium in which the cell grew inder nutritional stress. Steady-state parameters of semicon-finuous cultures conducted at 24-epibrassinolide concentra-ions from 10~16 M to 1CT9 M were evaluated. Typical effects of he agent that were observed both in standard and in diluted i [media were suppression of intracellular antibody level, increa- / ,ed value of mitochondrial membrane potential, higher frac- / ion of cells in the G(/Gl phase and lower fraction of cells in / he S phase. Alleviation of nutritional stress was observed in j (cultures conducted in diluted media. Viable cell density was j ignificantly higher at 24-epibrassinolide concentrations 10~13[ knd 10~12 M. Results of this exploratory study show that thej plant hormone 24-epibrassinolide may induce alterations of he cell division mechanism of the energetic metabolism, and of secreted protein synthesis in a mammalian cell line.
Workplace
Institute of Experimental Botany
Contact
David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing
2004
Number of the records: 1
This site uses cookies to make them easier to browse. Learn more about
how we use cookies.
