0172334 - UEB-Q 20033059 RIV CZ eng J - Journal Article
Burketová, Lenka - Štillerová, Kateřina - Feltlová, Marcela - Šindelářová, Milada
Immunohistological analysis of chemically induced proteins in sugar beet.
Biologia Plantarum. Roč. 47, č. 2 (2003), s. 243-251. ISSN 0006-3134. E-ISSN 1573-8264.
[Conference on Isotope Effect. Uppsala, 22.06.2003-27.06.2003]
R&D Projects: GA ČR GA522/00/0602
Institutional research plan: CEZ:AV0Z5038910
Keywords : immunolocalization * beta-1,3-glucanase * chitinase
Subject RIV: CE - Biochemistry
Impact factor: 0.919, year: 2003

Tissue-specific distribution of basic p-l,3-glucanase (Glu2), basic class II chitinase (Ch2), basic class IV chitinase (Ch4), and acidic class III chitinase (SE2) were examined both in leaves and roots of sugar beet treated with salicylic acid (SA), benzothiadiazole (BTH) and glycine betaine. Protein localization was monitored by immunohistological analysis using specific antibodies. BTH, SA as well as glycine betaine induced both Glu2 and chitinase isozymes in leaves and roots of treated plants. The enzymes were accumulated in extracellular space and cell walls. They were mostly deposited in parenchyma cells of leaves and cortex parenchyma and endodermis of roots. In leaf tissues, BTH and SA induced proteins more effectively than glycine betaine but the effect of glycine betaine in roots was as efficient as BTH and SA. Glycine betaine induced the formation of extracellular globuli containing Ch4. Induced proteins were spatially distributed over the whole plant regardless the site of the inducer application.
Permanent Link: http://hdl.handle.net/11104/0069373