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Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus
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SYSNO ASEP 0172316 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus Author(s) Čeřovská, Noemi (UEB-Q) RID, ORCID
Moravec, Tomáš (UEB-Q) RID, ORCID
Rosecká, Pavla (UEB-Q)
Dědič, P. (CZ)
Filigarová, Marie (UEB-Q)Source Title Journal of Phytopathology - Phytopathologische Zeitschrift. - : Wiley - ISSN 0931-1785
Roč. 151, č. 4 (2003), s. 195-200Number of pages 6 s. Language eng - English Country DE - Germany Keywords potato mop-top virus ; recombinant coat protein ; Escherichia Coli Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA522/01/1121 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z5038910 - UEB-Q Annotation The coat protein (CP) coding regions of two Czech Potato mop-top virus (PMTV) isolates were sequenced and shown to be identical. One, the Korneta isolate CP gene, was cloned in several expression vectors. The recombinant PMTV-CP was expressed in Escherichia coli and the purified recombinant protein was used to produce PMTV-specific polyclonal antibodies. The antiserum had a titre of 1 : 2000 in an indirect enzyme-linked immunosorbent assay (ELISA) and reacted specifically in immunoblotting and IPTA- ELISA (indirect plate-trapped antigen (PTA)-ELISA). Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2004
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