0172316 - UEB-Q 20033041 RIV DE eng J - Journal Article
Čeřovská, Noemi - Moravec, Tomáš - Rosecká, Pavla - Dědič, P. - Filigarová, Marie
Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus.
Journal of Phytopathology - Phytopathologische Zeitschrift. Roč. 151, č. 4 (2003), s. 195-200. ISSN 0931-1785. E-ISSN 1439-0434
R&D Projects: GA ČR GA522/01/1121
Institutional research plan: CEZ:AV0Z5038910
Keywords : potato mop-top virus * recombinant coat protein * Escherichia Coli
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.557, year: 2003

The coat protein (CP) coding regions of two Czech Potato mop-top virus (PMTV) isolates were sequenced and shown to be identical. One, the Korneta isolate CP gene, was cloned in several expression vectors. The recombinant PMTV-CP was expressed in Escherichia coli and the purified recombinant protein was used to produce PMTV-specific polyclonal antibodies. The antiserum had a titre of 1 : 2000 in an indirect enzyme-linked immunosorbent assay (ELISA) and reacted specifically in immunoblotting and IPTA- ELISA (indirect plate-trapped antigen (PTA)-ELISA).
Permanent Link: http://hdl.handle.net/11104/0069355