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DNA damage measured by the Comet assay in eight agronomic plants

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    SYSNO ASEP0172312
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleDNA damage measured by the Comet assay in eight agronomic plants
    Author(s) Gichner, Tomáš (UEB-Q)
    Mühlfeldová, Zdeňka (UEB-Q)
    Kim, J. K. (KR)
    Source TitleBiologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
    Roč. 47, č. 2 (2003), s. 185-188
    Number of pages4 s.
    Languageeng - English
    CountryCZ - Czech Republic
    KeywordsBeta vulgaris ; ethyl methanesulphonate ; Lens esculenta
    Subject RIVEB - Genetics ; Molecular Biology
    R&D ProjectsGA521/02/0400 GA ČR - Czech Science Foundation (CSF)
    LN00B030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    CEZAV0Z5038910 - UEB-Q
    AnnotationFor most crops growing in polluted areas or treated with agricultural chemicals, no genotoxicity assays are available. We have studied the possibility of using the alkaline protocol of the plant-based molecular assay - the Single Cell Gel Electrophoresis (SCGE) assay (also called Comet assay) as a method for detecting induced DNA damage in 8 agronomic important plants (ordered according to the diameter of the nuclei): sugar beet, alfalfa, tobacco, lentil, maize, potato, hard wheat, and bread wheat. The monofunctional alkylating agent ethyl methanesulphonate (EMS) was applied as a model genotoxic agent on young excised leaves of the tested crops for 18 h at 26 degrees C in the dark. With increasing concentrations of 2 to 10 mM EMS, the DNA damage, expressed by the averaged median tail moment values, significantly increased in nuclei of all crops studied. No correlation between the diameter of nuclei and sensitivity to EMS treat ment was observed. The data obtained demonstrate the feasibility of using the Comet assay for detecting induced DNA damage in crops.
    WorkplaceInstitute of Experimental Botany
    ContactDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Year of Publishing2004

Number of the records: 1  

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