0172281 - UEB-Q 20023066 RIV NL eng J - Journal Article
Vláčilová, K. - Ohri, D. - Vrána, Jan - Čihalíková, Jarmila - Doleželová, Marie - Kahl, G. - Doležel, Jaroslav
Development of flow cytogenetics and physical genome mapping in chickpea (Cicer arietinum L.).
Chromosome Research. Roč. 10, č. 8 (2002), s. 695-706. ISSN 0967-3849. E-ISSN 1573-6849
R&D Projects: GA AV ČR IAA6038204; GA AV ČR IBS5038104
Institutional research plan: CEZ:AV0Z5038910
Keywords : cell cycle * chickpea (Cicer arietinum) * human cytogenetics
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.828, year: 2002

Procedures for flow cytometric analysis and sorting of mitotic chromosomes (flow cytogenetics) have been developed for chickpea (Cicer arietinum). Suspensions of intact chromosomes were prepared from root tips treated to achieve a high degree of metaphase synchrony. The optimal protocol consisted of a treatment of roots with 2 mmol/L hydroxyurea for 18 h, a 4.5-h recovery in hydroxyurea-free medium, 2 h incubation with 10 micro mol/L oryzalin, and ice-water treatment overnight. This procedure resulted in an average metaphase index of 47
Permanent Link: http://hdl.handle.net/11104/0069320