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Plant cytokinin analogues with inhibitory activity on cyclin-dependent kinases exert their antiproliferative effect through induction of apoptosis initiated by the mitochondrial pathway: Determination by a multiparametric flow cytometric analysis
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SYSNO ASEP 0172279 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Plant cytokinin analogues with inhibitory activity on cyclin-dependent kinases exert their antiproliferative effect through induction of apoptosis initiated by the mitochondrial pathway: Determination by a multiparametric flow cytometric analysis Author(s) Vermeulen, K. (BE)
Strnad, Miroslav (UEB-Q) RID, ORCID
Havlíček, Libor (UEB-Q) RID, ORCID
Onckelen, H. (BE)
Lenjou, M. (BE)
Nijs, G. (BE)
Bockstaele, D. R. (BE)
Berneman, Z. N. (BE)Source Title Experimental Hematology. - : Elsevier - ISSN 0301-472X
Roč. 30, č. 10 (2002), s. 1107-1114Number of pages 8 s. Language eng - English Country US - United States Keywords PLASMA MEMBRANE ; PURINE ANALOGS ; CELLS Subject RIV EB - Genetics ; Molecular Biology R&D Projects OC 844.10 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z5038910 - UEB-Q Annotation Regulation of the cell cycle by cyclin-dependent kinase (CDK) activity occurs at multiple levels and is often altered in human cancers. Therefore, CDK activity has been targeted for drug discovery, and a number of small molecules have now been identified as CDK inhibitors. Plant cytokinin analogues with CDK inhibitory activity and antiproliferative effects were studied to characterize the cellular basis of the cytotoxic effect. The main cellular mechanism of the antiproliferative effect of plant cytokinin analogues with CDK inhibitory activity is the induction of apoptosis. The multiparametric flow cytometric technique allowed to follow the kinetics of various aspects of apoptotic cell changes and demonstrated that cytokinin analogue-induced apoptosis starts through the mitochondrial pathway. This technique could also become of value for the rapid screening of pro-apoptotic properties of chemotherapeutic compounds. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2003
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