0172258 - UEB-Q 20023043 RIV NL eng J - Journal Article
Doležal, Karel - Astot, C. - Hanuš, Jan - Holub, Jan - Peters, W. - Beck, E. - Strnad, Miroslav - Sandberg, G.
Identification of aromatic cytokinins in suspension cultured photoautotrophic cells of Chenopodium rubrum by capillary liquid chromatography/frit ů fast atom bombardment mass spectrometry.
Plant Growth Regulation. Roč. 36, č. 2 (2002), s. 181-189. ISSN 0167-6903. E-ISSN 1573-5087
R&D Projects: GA ČR GA522/01/0275; GA MŠMT OC 844.10; GA ČR GA301/02/0475
Institutional research plan: CEZ:AV0Z5038910
Keywords : Aromatic cytokinins * Capillary liquid chromatography/mass spectrometry * Chenopodium rubrum
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.850, year: 2002

Two previously unrecorded endogenous cytokinin metabolites, 6-[2-(4;-D-glucopyranosyloxy)benzylamino]purine and 6-[2-(4;-D-glucopyranosyloxy)benzylamino]-2-methylthiopurine, were identified, together with 6-benzylamino-9-L;-D-glucopyranosylpurine (BAP9G), from Chenopodium rubrum cells, grown autotrophically in suspension culture. The new metabolites belong to the aromatic class of cytokinins, in which an aromatic side chain is attached at the N6-position of the adenine species. The identification was performed by capillary-liquid chromatography/frit-fast atom bombardment - mass spectrometry (LC/FAB MS) after pre-column derivatisation and the structural elucidation was confirmed by organic synthesis. Cytokinin activity of the compounds was tested in an Amaranthus bioassay. The endogenous synthesis of the identified compounds was verified by in vivo deuterium labelling of the analysed cytokinin species, thereby for the first time providing absolute evidence for the endogenous origin of these compounds.
Permanent Link: http://hdl.handle.net/11104/0069297