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Polyclonal Antibodies to a Recombinant Coat Protein of Potato Virus A
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SYSNO ASEP 0172249 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Polyclonal Antibodies to a Recombinant Coat Protein of Potato Virus A Author(s) Čeřovská, Noemi (UEB-Q) RID, ORCID
Moravec, Tomáš (UEB-Q) RID, ORCID
Velemínský, Jiří (UEB-Q)Source Title Acta virologica - ISSN 0001-723X
Roč. 46, - (2002), s. 147-151Number of pages 5 s. Language eng - English Country CZ - Czech Republic Keywords Potato virus A ; recombinant coat protein ; Escherichia coli Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA310/00/0381 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z5038910 - UEB-Q Annotation Specific mouse antibodies against a recombinant coat protein (CP) of Potato virus A (PVA) were produced. The PVA CP gene was cloned in an expression vector. After expression in Escherichia coli the presence of the expressed CP was proved by Western blot analysis using polyclonal and monoclonal antibodies (MAbs). The expressed CP was purified by centrifugation in CsCl density gradient or on a sucrose cushion. The production of virus-like particles (VLPs) was proved by electron microscopy. The purified CP was used for preparation of a mouse antiserum which had a liter of 1:1024 in ELIS A and reacted specifically in Western blot analysis and indirect plate-trapped antigen ELISA (PTA-ELISA). Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2003
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