0172249 - UEB-Q 20023029 RIV CZ eng J - Journal Article
Čeřovská, Noemi - Moravec, Tomáš - Velemínský, Jiří
Polyclonal Antibodies to a Recombinant Coat Protein of Potato Virus A.
Acta virologica. Roč. 46, - (2002), s. 147-151. ISSN 0001-723X. E-ISSN 1336-2305
R&D Projects: GA ČR GA310/00/0381
Institutional research plan: CEZ:AV0Z5038910
Keywords : Potato virus A * recombinant coat protein * Escherichia coli
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.660, year: 2002

Specific mouse antibodies against a recombinant coat protein (CP) of Potato virus A (PVA) were produced. The PVA CP gene was cloned in an expression vector. After expression in Escherichia coli the presence of the expressed CP was proved by Western blot analysis using polyclonal and monoclonal antibodies (MAbs). The expressed CP was purified by centrifugation in CsCl density gradient or on a sucrose cushion. The production of virus-like particles (VLPs) was proved by electron microscopy. The purified CP was used for preparation of a mouse antiserum which had a liter of 1:1024 in ELIS A and reacted specifically in Western blot analysis and indirect plate-trapped antigen ELISA (PTA-ELISA).
Permanent Link: http://hdl.handle.net/11104/0069288