0172242 - UEB-Q 20023021 RIV DE eng J - Journal Article
Petrášek, Jan - Elčkner, Miroslav - Morris, David - Zažímalová, Eva
Auxin efflux carrier activity and auxin accumulation regulate cell division and polarity in tobacco cells.
Planta. Roč. 216, - (2002), s. 302-308. ISSN 0032-0935. E-ISSN 1432-2048
R&D Projects: GA ČR GA206/98/1510
Grant - others:INCO Copernicus(BE) IC15-CT98-0118
Institutional research plan: CEZ:AV0Z5038910
Keywords : Auxin carrier * 1,N,Naphthylphthalamic acid * Nicotiana (cell culture)
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.960, year: 2002

Division and growth of most types of in vitro-cultured plant cells require an external source of auxin. In such cultures, the ratio of external to internal auxin concentration is crucial for the regulation of the phases of the standard growth cycle. In this report the internal concentration of auxin in suspension-cultured cells of Nicotiana tabacum L., strain VBI-0, was manipulated either (i) by increasing 10-fold the normal concentration of 1-naphthaleneacetic acid (NAA) and 2,4-dichloro-phenoxyacetic acid in the external medium; or (ii) by addition 1-N-naphthylphthalamic acid (NPA; an inhibitor of auxin efflux and of auxin efflux carrier traffic). Both treatments delayed the onset of cell division for 6-7 days without loss of cell viability. In both cases, cell division activity subsequently resumed coincident with a reduction in the ability of cells to accumulate [3H]NAA from an external medium.
Permanent Link: http://hdl.handle.net/11104/0069281