0172241 - UEB-Q 20023020 RIV DE eng J - Journal Article
Petrášek, Jan - Březinová, Alena - Holík, Josef - Zažímalová, Eva
Excretion of cytokinins into the cultivation medium by suspension-cultured tobacco cells.
Plant Cell Reports. Roč. 21, - (2002), s. 97-104. ISSN 0721-7714. E-ISSN 1432-203X
R&D Projects: GA AV ČR IAA6038706; GA MŠMT ME 585
Institutional research plan: CEZ:AV0Z5038910
Keywords : Cytokinins * Plant cell cultures * Growth cycle
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.340, year: 2002

The dynamics of individual cytokinins were determined in both the cells and the cultivation medium during the subculture interval of cell suspension cultures of Nicotiana tabacum L., line VBI-0. The amounts of cytokinins detected in the cultivation medium were less than 1 pmol ml ' of suspension. In the late stationary phase, the levels of isopentenyladenosine, as well as that of dihydrozeatin and its riboside, increased significantly. However, when expressed per cell number, the levels of zeatin- and isopentenyladenine-type cytokinins in both the cells and medium were at a maximum at the beginning of the subculture interval and then gradually decreased. Cytokinins were excreted from the cells during the whole subcultivation period, and their concentrations in the cultivation medium were found to be approximately in proportion to their momentary levels inside the cells. The excretion might thus represent one of the mechanisms controlling endogenous cytokinin concentrations.
Permanent Link: http://hdl.handle.net/11104/0069280