Number of the records: 1
Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L)
- 1.
SYSNO ASEP 0172237 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L) Author(s) Doleželová, Marie (UEB-Q)
Vrána, Jan (UEB-Q) RID, ORCID
Čihalíková, Jarmila (UEB-Q) RID
Šimková, Hana (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCIDSource Title Theoretical and Applied Genetics. - : Springer - ISSN 0040-5752
Roč. 104, - (2002), s. 1362-1372Number of pages 11 s. Language eng - English Country DE - Germany Keywords Chromosome isolation ; Chromosome sorting ; Flow cytometry Subject RIV EB - Genetics ; Molecular Biology R&D Projects IAA6038204 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IBS5038104 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z5038910 - UEB-Q Annotation Previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. That study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. However, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3B could be sorted into a high-purity fraction. The present study aimed to identify wheat stocks that could be used to sort other chromosomes. An analysis of 58 varieties and landraces demonstrated a remarkable reproducibility and sensitivity of flow cytometry for the detection of numerical and structural chromosome changes. Changes in flow karyotype, diagnostic for the presence of the 1BL-1RS transloca-tion, have been found and lines from which translocation chromosomes 5BL-7BL and 4AL-4AS-5BL could be sorted have been identified. Furthermore, wheat lines have been identified which can be used for sorting chromosomes 4B, 4D, 5D and 6D. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2003
Number of the records: 1