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Animal Cell Technology - Basic and Applied Aspects
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SYSNO ASEP 0172229 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Antiproliferative and Growth-stimulating Activities of Synthetic Cytokinin Analogs Author(s) Franěk, František (UEB-Q)
Strnad, Miroslav (UEB-Q) RID, ORCID
Havlíček, Libor (UEB-Q) RID, ORCID
Siglerová, Věra (UEB-Q)Source Title Animal Cell Technology - Basic and Applied Aspects. - NL : Kluwer Academic Publishers, 2002 - ISBN 0-7923-1882-X Pages s. 315-319 Number of pages 5 s. Language eng - English Country NL - Netherlands Keywords Cytokinin Analogs ; Growth stimulating Subject RIV CE - Biochemistry R&D Projects OC 844.10 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA301/02/0475 GA ČR - Czech Science Foundation (CSF) GA303/02/0875 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z5038910 - UEB-Q Annotation Analogs of aromatic cytokinins, olomoucine, roscovitine, and bohemine, were screened for their capacity in supressing hybridoma cell growth and maintaining monoclonal antibody (MAb) production. Bohemine was selected as the most promising candidate for application. Addition of bohemine at concentrations in the range of 1-10 uM resulted in a short-term arrest of growth and of MAb secretion. The short-term suppression of cell functions was followed by a significant increase of specific growth rate and of specific production rate. At 3 uM bohemine concentration a moderate enhancement of MAb yield could be observed. The steady-state viable cell density values found in semicontinuous cultures showed stimulation of cell growth in the range of micromolar concentrations of bohemine, and inhibition of growth at higher concentrations. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2003
Number of the records: 1