0172229 - UEB-Q 20023011 RIV NL eng M - Monography Chapter
Franěk, František - Strnad, Miroslav - Havlíček, Libor - Siglerová, Věra
Antiproliferative and Growth-stimulating Activities of Synthetic Cytokinin Analogs.
Animal Cell Technology - Basic and Applied Aspects. NL: Kluwer Academic Publishers, 2002, s. 315-319. ISBN 0-7923-1882-X
R&D Projects: GA MŠMT OC 844.10; GA ČR GA301/02/0475; GA ČR GA303/02/0875
Institutional research plan: CEZ:AV0Z5038910
Keywords : Cytokinin Analogs * Growth stimulating
Subject RIV: CE - Biochemistry

Analogs of aromatic cytokinins, olomoucine, roscovitine, and bohemine, were screened for their capacity in supressing hybridoma cell growth and maintaining monoclonal antibody (MAb) production. Bohemine was selected as the most promising candidate for application. Addition of bohemine at concentrations in the range of 1-10 uM resulted in a short-term arrest of growth and of MAb secretion. The short-term suppression of cell functions was followed by a significant increase of specific growth rate and of specific production rate. At 3 uM bohemine concentration a moderate enhancement of MAb yield could be observed. The steady-state viable cell density values found in semicontinuous cultures showed stimulation of cell growth in the range of micromolar concentrations of bohemine, and inhibition of growth at higher concentrations.
Permanent Link: http://hdl.handle.net/11104/0069268