0172226 - UEB-Q 20023008 RIV DE eng J - Journal Article
Synková, Helena - Pospíšilová, Jana
n vitro precultivation of tobacco affects the response of antioxidative enzymes to ex vitro acclimation.
Journal of Plant Physiology. Roč. 159, - (2002), s. 781-789. ISSN 0176-1617. E-ISSN 1618-1328
R&D Projects: GA ČR GA522/02/1099; GA ČR GA206/01/1061
Institutional research plan: CEZ:AV0Z5038910
Keywords : abscisic acid * antioxidant enzymes * carbon dioxide
Subject RIV: ED - Physiology
Impact factor: 0.941, year: 2002

Both growth under elevated C02 (CE) conditions and/or treatment with abscisic acid (ABA) during acclimation to ex vitro conditions and precultivation in vitro in tightly closed glass vessels (G-plants) or ventilated Magenta boxes (M-plants) affected activities of antioxidative enzymes of tobacco 28 days after the transfer. Single treatment with 5|o.mol/L ABA immediately after transplantation caused an increase in net photosynthetic rate and in the content of chlorophyll a, and decreases in the activities of glutathione reductase, Mn-SOD, peroxidases, malic enzyme (ME), and glucose-6-phosphate dehydrogenase (G-6P-DH) in both types of plantlets. Contrary to this, CE effects were dependent on the plant origin and promoted activities of peroxidase, ME, and G6PDH were observed more in M-plants than in G-plants. Effects of a single ABA treatment lasted throughout the whole acclimation and alleviated transplantation shock more efficiently than CE, irrespective of plant precultivation. However, under combined treatment (CE+ABA) CE effects prevailed over ABA.
Permanent Link: http://hdl.handle.net/11104/0069265