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Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction
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SYSNO ASEP 0172223 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction Author(s) Dobrev, Petre (UEB-Q) RID, ORCID
Kamínek, Miroslav (UEB-Q) RID, ORCIDSource Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 950, č. 1 (2002), s. 21-29Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords cytokinins ; abscisic acid ; extraction Subject RIV EF - Botanics R&D Projects GA206/02/0967 GA ČR - Czech Science Foundation (CSF) GA522/02/0530 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z5038910 - UEB-Q Annotation A method for separation of cytokinins from auxin and abscisic acid, which allows further separation ofcylokinin ribotides from cytokinin bases, ribosidcs und glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationie cylokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluled by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of (he method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (R1A) or I1PLC-MS. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2003
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