0170576 - UEK-B 20013036 RIV GB eng J - Journal Article
Šafaříková, Miroslava - Šafařík, Ivo
Immunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables.
Letters in Applied Microbiology. Roč. 33, - (2001), s. 36-39. ISSN 0266-8254. E-ISSN 1472-765X
R&D Projects: GA ČR GA203/98/1145
Institutional research plan: CEZ:AV0Z6087904
Keywords : immunomagnetic * E. coli
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.151, year: 2001

Raw vegetable and fruit products have been increasingly connected with human infections with Shiga toxin-producing Escherichia coli. This study evaluated the isolation and detection of E. coli O26, O111 and O157 inoculated into vegetable samples using immunomagnetic particles. Standard cultivation and immunomagnetic separation (IMS) procedures were compared. It was found that immunomagnetic particles very efficiently concentrate E. coli cells, detecting significantly more bacteria then with standard cultivation procedures. E. coli O111and O157 were detected in 93-100 % of inoculated samples using IMS, but only 36-53 % samples after plating were found positive. In the case of E. coli O26, 93 % samples after direct plating were positive compared with 100 % positive samples after IMS; the number of presumptive colonies was significantly higher after IMS.
Permanent Link: http://hdl.handle.net/11104/0067660