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Molecular identification of three crested newt species (Triturus cristatus superspecies) by RAPD markers
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SYSNO ASEP 0164544 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Molecular identification of three crested newt species (Triturus cristatus superspecies) by RAPD markers Author(s) Mikulíček, P. (CZ)
Piálek, Jaroslav (UBO-W) RID, ORCID, SAISource Title Amphibia-Reptilia. - : Brill - ISSN 0173-5373
Roč. 24, č. 2 (2003), s. 201-207Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords Triturus cristatus ; genetic variation Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA206/98/0115 GA ČR - Czech Science Foundation (CSF) GA206/01/0695 GA ČR - Czech Science Foundation (CSF) KSK6005114 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z6093917 - UBO-W MSM 113100004 DOI 10.1163/156853803322390444 Annotation We have applied Random amplified polymorphic DNA (RAPD) analysis to three newt species T. cristatus, T. dobrogicus and T. carnifex occurring in the Czech Republic and Slovakia to identify species-specific molecular markers, potentially useful for newt determination. Out of twenty randomly used RAPD primers, just one did not amplify any DNA fragment. Amplification reactions from the other 19 primers yielded 2 to 22 bands per primer. The total number of scored bands was 202. The average number of bands per primer and individual was 5.3. While the similarity indexes among individuals within populations (0.94 ů 0.99) and between populations within species (0.81 ů 0.98) were very high, between-species similarity was relatively low with the lowest value for T. carnifex and T. cristatus (0.49) and the highest value for T. dobrogicus and T. cristatus (0.53). Although nineteen primers amplified DNA fragments, only twelve generated clear highly reproducible species-specific bands. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2004
Number of the records: 1