0162492 - UIACH-O 20033052 RIV FI eng C - Conference Paper (international conference)
Klepárník, Karel
Alkaline Capillary Electrophoresis.
ITP 2002. The 13th International Symposium on Capillary Electroseparation Techniques. Program and Book of Abstracts. Helsinki, 2002, s. 33.
[ITP 2002. International Symposium on Capillary Electroseparation Techniques /13./. Helsinki (FI), 01.09.2002-04.09.2002]
R&D Projects: GA ČR GA203/00/0772; GA ČR GA303/00/0928
Institutional research plan: CEZ:AV0Z4031919
Keywords : single strand conformation polymorphism * single cell analysis
Subject RIV: CB - Analytical Chemistry, Separation

The size-based separations of single-stranded DNA fragments under denaturing conditions are more selective than the separations of their double-stranded analogs. With respect to the denaturing ability, NaOH is probably the strongest denaturing agent. Thus, even the DNA fragments with GC rich regions, which are resistant to the denaturing in urea, dissociate completely at pH over 12.
Permanent Link: http://hdl.handle.net/11104/0059805