0162470 - UIACH-O 20033033 RIV CZ eng C - Conference Paper (international conference)
Horká, Marie - Růžička, F. - Šlais, Karel
Dynamic modification of microorganisms by pyrene-butanoate for fluorometric detection in capillary electrokinetic techniques.
FEMS Congress of European Microbiologists. Abstract book. Delft: Elsevier, Federation of European Microbiological Societies, 2003, s. 283.
[FEMS Congress of European Microbiologists /1./. Ljubljana (SI), 29.06.2003-03.07.2003]
R&D Projects: GA AV ČR IAA4031901; GA AV ČR IAA4031302; GA ČR GA203/02/1447
Institutional research plan: CEZ:AV0Z4031919
Keywords : capillary electrokinetic techniques * dynamic modification of microorganisms * fluorometric detection
Subject RIV: CB - Analytical Chemistry, Separation

Pyrenebutanoate and non-ionogenic tenside on the basis of pyrenebutanoate as the fluorescent compounds were used as buffer additives in capillary zone electrophoresis or capillary isoelectric focusing, respectively, for a dynamic modification of the microbial samples. Using the deuterium lamp UV excitation for the on column fluorometric detection, the minimum detectable amount of the microorganisms in order of ones to tens of cells sampled on the separation capillary was achieved.
Permanent Link: http://hdl.handle.net/11104/0059784