0162465 - UIACH-O 20033028 RIV FR eng C - Conference Paper (international conference)
Šťastná, Miroslava - Šlais, Karel
Dynamics of gel IEF with ampholytic dyes monitored by camera in real-time.
27th Symposium on High Performance Liquid Phase Separations and Related Techniques. Program & Abstracts. Nice, 2003, s. 129.
[HPLC 2003. Symposium on High Performance Liquid Phase Separations and Related Techniques /27./. Nice (CZ), 15.06.2003-19.06.2003]
R&D Projects: GA AV ČR IBS4031201
Institutional research plan: CEZ:AV0Z4031919
Keywords : Dynamics * gel IEF * real-time
Subject RIV: CB - Analytical Chemistry, Separation

Dynamics of IEF was examined by using low molecular mass colored ampholytes (pI markers) in polyacrylamide slab gels. The pH gradient was created by the simple buffers and compared to IEF performed with synthetic carrier ampholytes. The experimental arrangement with electrodes in direct contact with gel was applied. The evolution of electrofocusing was monitored by CCD camera in real-time and video record was evaluated.
Permanent Link: http://hdl.handle.net/11104/0059780