0162450 - UIACH-O 20033013 RIV DE eng J - Journal Article
Horká, Marie - Planeta, Josef - Růžička, F. - Šlais, Karel
Sol-gel column technology for capillary isoelectric focusing of microorganisms and biopolymers with UV or fluorometric detection.
Electrophoresis. Roč. 24, č. 9 (2003), s. 1383-1390. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA AV ČR IAA4031901; GA AV ČR IAA4031302; GA ČR GA203/02/1447
Institutional research plan: CEZ:AV0Z4031919
Keywords : sol-gel CIEF * microorganisms
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.040, year: 2003

The sol-gel surface modification is used for capillary isoelectric focusing of microorganisms and biopolymers. The coating strongly decreases the electroosmotic flow so that it enables the use of the short capillaries down to 100 mm in the separation length. The examples of capillary isoelectric focusing of the low-molecular-mass pI markers and mixed cultures of microbial populations of Escherichia coli, Candida albicans, Staphylococcus epidermidis and Enteroccocus faecalis with UV detection are shown. It is possible to quantify bacterial cells according their peak areas; the minimum detectable number of microbial cells was 5 x 102 1 x 103. The compatibility of sol-gel capillaries with sensitive fluorometric detection of fluorescent pI markers together with fluorescein labeled proteins is demonstrated.
Permanent Link: http://hdl.handle.net/11104/0059765