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Determination of saccharides in fruit juices by capillary electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry

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    SYSNO ASEP0162345
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleDetermination of saccharides in fruit juices by capillary electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    Author(s) Žídková, Jitka (UIACH-O)
    Chmelík, Josef (UIACH-O)
    Source TitleJournal of Mass Spectrometry. - : Wiley - ISSN 1076-5174
    Roč. 36, č. 4 (2001), s. 417-421
    Number of pages5 s.
    Languageeng - English
    CountryGB - United Kingdom
    Keywordsfruit juices ; adulteration ; matrix-assisted laser deasorption
    Subject RIVCB - Analytical Chemistry, Separation
    R&D ProjectsEP9410 GA MZe - Ministry of Agriculture (MZe)
    CEZAV0Z4031919 - UIACH-O
    AnnotationA fast method for the detection of cheap sweeteners is presented. Detecting the adulteration of foods rich in carbohydrates is complicated by the presence of variety of commercial sweeteners that are designed to match exactly the major carbohydrate profiles of these foods. Electrophoretic and mass spectrometric assays for the determination of fruit juice authenticity were developed. Capillary zone electrophoresis with indirect detection was employed to detect adulteration of juice demonstrated by the ratio of the concentrations of major low molecular mass saccharides (glucose, fructose and sucrose). Traces of oligosaccharides, which are not present in the sugar profiles of citrus but are present in inexpensive sweeteners, were evaluated as the other group of target compounds. The fast determination of oligomeric starch hydrolysates in a complex matrix was tested by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and applied to orange juice.
    WorkplaceInstitute of Analytical Chemistry
    ContactIveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
    Year of Publishing2002

Number of the records: 1  

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