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Dynamic modification of proteins for fluorometric detection in CZE
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SYSNO ASEP 0162299 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Dynamic modification of proteins for fluorometric detection in CZE Author(s) Horká, Marie (UIACH-O) RID, ORCID
Šlais, Karel (UIACH-O) RID, ORCIDSource Title 2nd International Symposium Separations in the BioSciences. SBS 2001. - Praha, 2001 - ISBN 80-7080-437-8 Pages s. 91 Number of pages 1 s. Action International Symposium Separations in the BioSciences /2./ Event date 17.09.2001-20.09.2001 VEvent location Praha Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords capillary zone electrophoresis ; fluorometric detection ; dynamic modification Subject RIV CB - Analytical Chemistry, Separation R&D Projects IAA4031901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z4031919 - UIACH-O Annotation The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2002
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